PTG Assembly Designer
Design polycistronic tRNA-gRNA assemblies for CRISPR multiplex editing
How to Use
- Select the number of spacers for your assembly (2–9 spacers)
- Choose the appropriate gRNA scaffold type for your plant system
- Select the Terminal enzyme - used for first & last module only (FokI is default, and supports 11 others)
- Select the Golden Gate enzyme - joins all internal modules (BsaI is default, and supports 11 others)
- Enter your 20 bp spacer sequences (only A, T, G, C bases allowed)
- Click Generate Assembly to compute primer and module designs
- View the full results report, which opens in a new browser tab
Tip: Use the Load Example button to see sample sequences and explore a pre-filled assembly design.
- Automated primer design for all modules
- Enzymatic Golden Gate cloning with 12 IIs
- Supports monocot & dicot plant systems
- Excel / CSV export ready results